Quantifying Phenolic and Flavonoid Compounds

Briefly, the total phenolic contents of each ethanolic extract or serial concentrations of GA (standard) were mixed with Folin–Ciocalteau reagent and sodium carbonate. Phenolic concentration (mg/100 g plant tissue) was estimated using the standard curve of GA^{30 (link)}.
The total flavonoid contents of each ethanolic extract or serial concentrations of RU (standard) were mixed with sodium nitrite and aluminum chloride solution. Flavonoid concentration (mg/100 g plant tissue) was estimated using the standard curve of RU^{31 (link)}.

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Ahmad G.M., Abu Serie M.M., Abdel-Latif M.S., Ghoneem T., Ghareeb D.A, & Yacout G.A. (2023). Potential anti-proliferative activity of Salix mucronata and Triticum spelta plant extracts on liver and colorectal cancer cell lines. Scientific Reports, 13, 3815.

Publication 2023

Aluminum chloride Ethanolic Flavonoid Folin ciocalteau reagent Plant Sodium carbonate Sodium nitrite Tissue

Corresponding Organization :

Other organizations : Pharos University in Alexandria, City of Scientific Research and Technological Applications, Alexandria University

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Variable analysis

independent variables

Ethanolic extracts
Serial concentrations of GA (standard)
Serial concentrations of RU (standard)

dependent variables

Phenolic concentration (mg/100 g plant tissue)
Flavonoid concentration (mg/100 g plant tissue)

control variables

Folin–Ciocalteau reagent
Sodium carbonate
Sodium nitrite
Aluminum chloride solution

controls

Positive control: Standard curve of GA
Positive control: Standard curve of RU

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