Topical Treatments for Skin Inflammation

For inhibition of glucose transport, 1 mg/ml WZB-117 dissolved in acetone (Sigma-Aldrich) or vehicle (100% acetone) was applied topically once daily onto the ears of female ft/ft mice for 9 days according to previous work (Zhang et al., 2018 (link)). The epidermis was collected 6 hours after the last treatment. Psoriasis-like skin inflammation was induced in adult, age-matched, female C57BL/6 mice by topical application of a daily dose of 12.5 mg of Aldara cream (Meda Pharma GmbH, Frankfurt, Germany), containing 5% IMQ onto ears for 5 days (van der Fits et al., 2009 (link)). Control mice were topically treated with petroleum jelly. The epidermis was collected 24 hours after the last treatment. To induce ADL, 45 μM MC903 (Sigma Aldrich) in 12.5 μl ethanol was topically applied onto both sides of the ears of female C57BL/6 mice daily for 9 days consecutively (Moosbrugger-Martinz et al., 2017 (link)). Control mice were topically treated with ethanol (vehicle). The epidermis was collected 24 hours after the last treatment.