Synthesis and Purification of AWRK6 Peptide

The peptide AWRK6 was chemically synthesized using Fmoc solid-phase synthesis method and purified using HPLC for a purity of higher than 95%, followed by verification using HPLC-MS (GL Biochem, Shanghai, China) [10 (link)]. The obtained peptide was lyophilized for storage. PMB and heparin sodium were purchased from Meilunbio (Dalian, China). Methanol and acetonitrile (ACN) were purchased from Sigma-Aldrich (Shanghai, China). Formic acid (FA) was obtained from Mreda (Beijing, China) and dichloromethane was purchased from Aibi (Shanghai, China). All chemicals used were in HPLC grade. Ultrapure water was prepared using a Milli-Q water purifying system (Millipore, MA, USA).