Primary rat cortical neuronal cultures were treated with a combination of 100 μM 4-aminopyridine (4AP), 50 μM bicuculline, and 50 μM forskolin (hereafter abbreviated as 4BF) for 8 h to induce neural network activity as previously described [4 (link)]. This protocol has been previously reported to induce pharmacological LTP [66 (link),67 (link)].
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