Characterization of Human LAD Cell Lines

All human LAD cell lines were obtained from the Hamon Cancer Center Collection (UT Southwestern Medical Center, UTSW), were DNA fingerprinted with a PowerPlex 1.2 kit (Promega) and tested for mycoplasma using e-Myco kit (Boca Scientific). The cell lines were generated between 1979 and 2007. Cells were maintained in RPMI-1640 (Life Technologies) with 5% fetal bovine serum (FBS). 808-T3 and Green-Go [53 (link)] cell lines were kind gifts from Dr David McFadden (UTSW) and Dr Tyler Jacks (MIT), respectively, and were maintained in DMEM (Life Technologies) with 10% FBS. All cells were maintained at 37 °C, with 5% CO2, and under humidified conditions.