Immunohistochemical Staining of Ki67 in Tumor Tissue

Paraffin sections (5 μm) from tumor tissue samples were deparaffinized in 100% xylene and rehydrated with a decreasing ethanol series and then water. Antigen retrieval was conducted in 0.01 mol/L sodium citrate buffer (pH 6.0) at 100 °C for 10 min. Immunohistochemistry assay was performed as described previously [22 (link)] using super-sensitive horseradish peroxidase immunohistochemistry kit (Sangon Biotech, Shanghai, China) per the manufacturer’s instructions, and the anti-Ki67 antibody (sc-23900, Santa Cruz) was used.

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Luo K., Geng J., Zhang Q., Xu Y., Zhou X., Huang Z., Shi K.Q., Pan C, & Wu J. (2019). LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer. Journal of Experimental & Clinical Cancer Research : CR, 38, 249.

Publication 2019

super-sensitive horseradish peroxidase immunohistochemistry kit anti-Ki67 antibody (sc-23900,

Anti antibody Antigen Assay Buffer Ethanol Horseradish peroxidase Immunohistochemistry Paraffin Sodium citrate Tissue Tumor Xylene

Corresponding Organization :

Other organizations : Wenzhou Medical University, Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University

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Variable analysis

independent variables

Deparaffinization in 100% xylene
Rehydration with decreasing ethanol series
Antigen retrieval in 0.01 mol/L sodium citrate buffer (pH 6.0) at 100 °C for 10 min

dependent variables

Immunohistochemistry assay results

control variables

Tissue sample (tumor tissue)
Paraffin sections (5 μm)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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