Western Blotting Quantification of MMPs

Western blotting was performed as previously described [12 (link)]. Antibodies against MMP-9 (polyclonal antibody, Dako, Trappes. France), MMP-3 (clone 552A4, Oncogene research Product, Boston, Mass) and TIMP-1 (clone 7-6C1; Oncogene Research Products) were used.
Tspan8 was detected using a mouse monoclonal anti-Tspan8 antibody (TS29 clone [12 (link),18 (link),19 (link),20 (link),21 (link)]. Western blot quantifications were performed using ImageJ software. At least three independent biological replicates were performed.

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El Kharbili M., Cario M., Béchetoille N., Pain C., Boucheix C., Degoul F., Masse I, & Berthier-Vergnes O. (2020). Tspan8 Drives Melanoma Dermal Invasion by Promoting ProMMP-9 Activation and Basement Membrane Proteolysis in a Keratinocyte-Dependent Manner. Cancers, 12(5), 1297.

Publication 2020

MMP-9

Antibodies Antibody Biological Clone Mmp 3 Mmp 9 Monoclonal antibody Mouse Oncogene products Timp 1 Western blot

Corresponding Organization :

Other organizations : Centre National de la Recherche Scientifique, Laboratoire de Biologie Moléculaire de la Cellule, University of Colorado Anschutz Medical Campus, Université Claude Bernard Lyon 1, Inserm, Bordeaux Population Health, Université Paris-Sud, Université Clermont Auvergne, Centre de Recherche en Cancérologie de Lyon

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Variable analysis

independent variables

MMP-9 (polyclonal antibody, Dako, Trappes. France)
MMP-3 (clone 552A4, Oncogene research Product, Boston, Mass)
TIMP-1 (clone 7-6C1; Oncogene Research Products)
Tspan8 (mouse monoclonal anti-Tspan8 antibody, TS29 clone)

dependent variables

Protein expression levels of MMP-9, MMP-3, TIMP-1, and Tspan8

control variables

Western blotting procedure, as previously described [12]

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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