LT-ApoE−/− mice were fed a western diet for 15 weeks. For the last 6 weeks, mice were injected intraperitoneal with 100 μg of anti-ICOSL (clone HK5.3) or isotype control (Rat IgG2a) (BioXcell, West Lebanon, NH) twice a week, similar to Choi et al.30 (link). Mice were sacrificed and Tfh populations were assessed in aorta, peri-aortic LNs and spleen as described earlier.
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