Lipid-based Anti-microRNA103/107 Delivery

Anti-microRNA103/107 (5′-rUrCrArUrArGrCrCrCrUrGrUrArCrArArUrGrCrUrGrCrU-3′) and (5′-/5RhoR-XN/rUrCrArUrArGrCrCrCrUrGrUrArCrArArUrGrCrUrGrCrU-3′), namely anti-miR103/107, was synthesized by Tema Ricerca s.r.l. (Bologna, Italy). 1,2-dioleyl-3-dimethylammonium propane (DODAP), N-palmitoyl-sphingosine-1-succinyl[methoxy(polyethylene glycol)2000] (PEG2000-Cer16) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]-Maleimide (DSPE-PEG-Mal) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium salt) were obtained by Avanti Polar Lipids. Disteroylphosphatidylcholine (DSPC) was kindly offered from Lipoid GmbH (Cam, Switzerland). Cholesterol (CHOL), human transferrin (Tf), ammonium ferrithiocyanate, sodium borate, sodium chloride, sodium citrate, sodium phosphate, HEPES, ethylenediaminetetraacetic acid (EDTA), citric acid, Sepharose G-25, and 2-iminothiolane (Traut’s reagent) were purchased from Sigma-Aldrich (USA). Ethanol and other solvents were obtained by Exacta Optech (Italy).

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Cepparulo P., Cuomo O., Campani V., Vinciguerra A., Sisalli M.J., Nele V., Anzilotti S., Valsecchi V., Casamassa A., Brancaccio P., Scorziello A., De Rosa G., Annunziato L, & Pignataro G. (2024). Anti-miRNA103/107 encapsulated in transferrin-conjugated lipid nanoparticles crosses blood-brain barrier and reduces brain ischemic damage. Molecular Therapy. Nucleic Acids, 35(1), 102131.

Publication 2024

PEG2000-Cer16 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) Disteroylphosphatidylcholine (DSPC) sodium borate sodium citrate HEPES 2-iminothiolane

Corresponding Organization :

Other organizations : University of Naples Federico II, Marche Polytechnic University, University of Sannio

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Variable analysis

independent variables

Anti-microRNA103/107 (5′-rUrCrArUrArGrCrCrCrUrGrUrArCrArArUrGrCrUrGrCrU-3′)
(5′-/5RhoR-XN/rUrCrArUrArGrCrCrCrUrGrUrArCrArArUrGrCrUrGrCrU-3′)

dependent variables

Not explicitly mentioned

control variables

1,2-dioleyl-3-dimethylammonium propane (DODAP)
N-palmitoyl-sphingosine-1-succinyl[methoxy(polyethylene glycol)2000] (PEG2000-Cer16)
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]-Maleimide (DSPE-PEG-Mal)
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium salt)
Disteroylphosphatidylcholine (DSPC)
Cholesterol (CHOL)
Human transferrin (Tf)
Ammonium ferrithiocyanate
Sodium borate
Sodium chloride
Sodium citrate
Sodium phosphate
HEPES
Ethylenediaminetetraacetic acid (EDTA)
Citric acid
Sepharose G-25
2-iminothiolane (Traut's reagent)
Ethanol and other solvents

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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