CD Spectroscopy of Peptides in Bacterial Membranes

CD spectra were recorded as described earlier^{45 (link)} on a Jasco 810 (Japan) dichrograph in quartz suprasil cells (Hellma) with an optical path of 1 mm using 40 µM peptide in solution in 30% isopropanol with 4% SDS (to mimic bacterial membranes) or without SDS. Spectra were obtained as before from 3 accumulations between 190 and 260 nm with a data pitch of 0.5 nm, a bandwidth of 1 nm and a standard sensitivity^{45 (link)}. The crude spectra were then converted in molar ellipticity per residue and processed using CDPro software package and the programs CDSSTR, CONTINLL and SELCON3^{50 (link)}.

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Olvera M.R., Garai P., Mongin G., Vivès E., Gannoun-Zaki L, & Blanc-Potard A.B. (2019). Synthetic hydrophobic peptides derived from MgtR weaken Salmonella pathogenicity and work with a different mode of action than endogenously produced peptides. Scientific Reports, 9, 15253.

Publication 2019

quartz suprasil cells

Bacterial Cells Isopropanol Membranes Molar Peptide Quartz

Corresponding Organization :

Other organizations : Laboratory of Pathogens and Host Immunity, Université de Montpellier, Centre National de la Recherche Scientifique, Centre de Recherche en Biologie cellulaire de Montpellier

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Variable analysis

independent variables

Presence or absence of SDS in the solution

dependent variables

CD spectra

control variables

Peptide concentration (40 μM)
Solvent (30% isopropanol)
Quartz suprasil cells with 1 mm optical path
CD spectrometer (Jasco 810)
Scan parameters (190-260 nm, 0.5 nm data pitch, 1 nm bandwidth, standard sensitivity)

controls

Positive control: CD spectra recorded in the presence of SDS to mimic bacterial membranes
Negative control: CD spectra recorded in the absence of SDS

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