The interaction between biotinylated or naturally secreted amyloid-β oligomers and EphB2 was assessed under cell-free conditions and in neuronal cultures of primary neurons or HEK cells by pull-down with avidin agarose beads43 (link) or immunoprecipitation and western blot44 (link). EphB2 and NR1 levels in brain tissues or neuronal cultures were determined by immunoprecipitation and western blot or western blot alone44 (link). Corresponding transcripts were measured by quantitative polymerase chain reaction with reverse transcription (RT-qPCR). Fos expression in neuronal cultures was determined by western blot44 (link). Field recordings8 (link) or whole-cell patch-clamp recordings45 (link) from acute hippocampal slices were used to determine synaptic strength (fEPSP I/O relationships; mediated by either AMPA receptors or NMDA receptors), synaptic plasticity (LTP), and NMDA-receptor:AMPA-receptor ratios of EPSCs at the medial perforant path to dentate gyrus granule cell synapses. Learning and memory were assessed in the Morris water maze, novel object recognition test, novel place recognition test, and passive avoidance test46 (link)–49 (link). Amyloid-β levels in the dentate gyrus of hAPP-J20 mice were determined by ELISA50 .