Comprehensive Immunophenotyping of Murine Hematopoietic Cells
Corresponding Organization :
Other organizations : Instituto de Investigaciones Biomédicas Sols-Morreale, Autonomous University of Madrid, Consejo Superior de Investigaciones Científicas
Protocol cited in 1 other protocol
Variable analysis
- Cell type (blood, BM cells, and splenocytes)
- Cell surface marker expression
- Cell viability (live and dead cells)
- Apoptosis
- Cell cycle
- Cell proliferation
- Cell number (0.5–2 × 10^6 cells)
- Staining duration (20 min)
- Staining temperature (room temperature)
- Washing procedure (twice with buffer A)
- Flow cytometer voltage settings (using unstained cells as negative control)
- Compensation (using single-colour positive controls)
- Absolute cell number calculation (using Perfect-Count Microspheres)
- Single-colour positive controls for adjusting compensation
- Unstained cells for establishing flow cytometer voltage settings
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