Male BALB/c mice (5–6 weeks) weighing around 25–30 g were purchased from Orient Bio (Seoul, South Korea) and housed under room temperature 22 ± 2 °C, 55 ± 5% humidity with 12 h light–dark cycle condition in the animal breeding center of Advanced Bio convergence center of Pohang Technopark foundation, South Korea. All the mice were provided with free access to drinking water and rodent food. Before the start of the study, we also ascertained that the experiments related to animal use were conducted in accordance with the Pohang Technopark Laboratory Ethics Committee (approval number ABCC2018004) after obtaining approval on 30 June 2018.
After one week of acclimatization, healthy mice were randomly allocated into six mice per group. For an acute liver injury model (n = 6/group), all mice were intraperitoneally injected with CCl4 (1 mL/kg) three times a week, except for control groups [42 (link)]. 5-DN mice groups were orally administered with 1 and 2 mg/kg every day until the end of the experiment. On the 15th day, mice were anesthetized with isoflurane, and the blood was drawn through cardiac puncture for biochemical analysis. Liver was weighed, measured, and collected for molecular and histological studies.
After one week of acclimatization, healthy mice were randomly allocated into six mice per group. For an acute liver injury model (n = 6/group), all mice were intraperitoneally injected with CCl4 (1 mL/kg) three times a week, except for control groups [42 (link)]. 5-DN mice groups were orally administered with 1 and 2 mg/kg every day until the end of the experiment. On the 15th day, mice were anesthetized with isoflurane, and the blood was drawn through cardiac puncture for biochemical analysis. Liver was weighed, measured, and collected for molecular and histological studies.
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