Hydrodynamic Injection for Liver Cancer

The FVB mice (8 weeks) were purchased from Westlake University (Zhejiang, China). Plasmids for injection were harvested using the Endotoxin Free Maxiprep kit (Qiagen) and delivered to the FVB mice as a mixture using the hydrodynamic tail vein injection for HCC formation as described before (Song et al., 2017 (link)). Dosages of these plasmids were: px330-U6-sgP53 (mouse) 20 μg, pT3-EF1α-c-Myc (human) 5 μg, pCMV-sleeping beauty transposase 2 μg. The daily abdominal palpation of mice was observed, and the mice were then sacrificed when they suffered from high burdens of liver tumors. The mice were raised according to the protocols approved by the Institutional Animal Care and Use Committee of Westlake University. The researcher followed the standard biosecurity and institutional safety procedures in this study.