Bovine serum albumin (BSA), dimethyl sulfoxide (DMSO), fibrinogen,
thrombin, and Tyrode’s salt solution were purchased from Sigma-Aldrich
(St. Louis, MO, USA). Adenosine diphosphate (ADP) was purchased from Chrono-Log
(Havertown, PA, USA). Paraformaldehyde (PFA) was purchased from Electron
Microscopy Sciences (Hatfield, PA, USA). Prostaglandin I2(PGI2) was purchased from Cayman Chemical (Ann Arbor, MI, USA).
The following Hsp inhibitors were kindly provided to us by several
collaborators: C86 (Hsp40 inhibitor; Jane Trepel, Developmental Therapeutics
Branch, NCI, Bethesda, MD, USA)[21 (link)], JG98
(Hsp70 inhibitor; Jason Gestwicki, University of California San Francisco, San
Francisco, CA, USA)[23 ], Kung65 (Grp94
inhibitor; Brian Blagg, University of Notre Dame, Notre Dame, IN, USA), and
TAS116 (Hsp90 inhibitor; Taiho Pharmaceutical Co., Tsukuba, Ibaraki,
Japan)[24 (link)]. Phalloidin AF488, and
AlexaFluor AF594 were purchased from Invitrogen Life Technologies (Carlsbad, CA,
USA). The flow cytometry antibodies CD61 AF647, CD62P BV421 (also known as
P-selectin), PAC-1 AF647 (the activated form of glycoprotein IIb/IIIa;
GPIIb/IIIa), and Annexin V Pacific Blue were purchased from Bio-Rad (Hercules,
CA, USA), BD Biosciences (San Jose, CA, USA), BioLegend (San Diego, CA, USA),
and Invitrogen Life Technologies (Carlsbad, CA, USA), respectively.
thrombin, and Tyrode’s salt solution were purchased from Sigma-Aldrich
(St. Louis, MO, USA). Adenosine diphosphate (ADP) was purchased from Chrono-Log
(Havertown, PA, USA). Paraformaldehyde (PFA) was purchased from Electron
Microscopy Sciences (Hatfield, PA, USA). Prostaglandin I2(PGI2) was purchased from Cayman Chemical (Ann Arbor, MI, USA).
The following Hsp inhibitors were kindly provided to us by several
collaborators: C86 (Hsp40 inhibitor; Jane Trepel, Developmental Therapeutics
Branch, NCI, Bethesda, MD, USA)[21 (link)], JG98
(Hsp70 inhibitor; Jason Gestwicki, University of California San Francisco, San
Francisco, CA, USA)[23 ], Kung65 (Grp94
inhibitor; Brian Blagg, University of Notre Dame, Notre Dame, IN, USA), and
TAS116 (Hsp90 inhibitor; Taiho Pharmaceutical Co., Tsukuba, Ibaraki,
Japan)[24 (link)]. Phalloidin AF488, and
AlexaFluor AF594 were purchased from Invitrogen Life Technologies (Carlsbad, CA,
USA). The flow cytometry antibodies CD61 AF647, CD62P BV421 (also known as
P-selectin), PAC-1 AF647 (the activated form of glycoprotein IIb/IIIa;
GPIIb/IIIa), and Annexin V Pacific Blue were purchased from Bio-Rad (Hercules,
CA, USA), BD Biosciences (San Jose, CA, USA), BioLegend (San Diego, CA, USA),
and Invitrogen Life Technologies (Carlsbad, CA, USA), respectively.
