Western Blot Analysis of Cell Lysates

Western blotting of whole cell lysates was carried out as previously described.^{22 (link),23 (link)} Antibodies against pY419 Src, total Src, and p85 were purchased from Cell Signaling Technologies (Danvers, MA), for pY512 ICAM-1, total ICAM-1, glyceraldehyde 3-phosphate dehydrogenase, and TNF-R1 (both rabbit and mouse origin) from Santa Cruz Biotechnology, and for pY14 caveolin-1, total caveolin-1, and (β-actin from BD Biosciences (Franklin Lakes, NJ).

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Piegeler T., Votta-Velis E.G., Bakhshi F.R., Mao M., Carnegie G., Bonini M.G., Schwartz D.E., Borgeat A., Beck-Schimmer B, & Minshall R.D. (2014). Endothelial Barrier Protection by Local Anesthetics: Ropivacaine and Lidocaine Block Tumor Necrosis Factor-α–induced Endothelial Cell Src Activation. Anesthesiology, 120(6), 1414-1428.

Publication 2014

glyceraldehyde 3-phosphate dehydrogenase TNF-R1 pY14 caveolin-1 total caveolin-1 (β-actin

Actin Antibodies Caveolin 1 Cell Glyceraldehyde 3 phosphate dehydrogenase Icam 1 Mouse Rabbit Tnf r1

Corresponding Organization :

Other organizations : University of Illinois Hospital & Health Sciences System, University of Illinois Chicago, University of Zurich

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Phosphorylation of Src (pY419 Src)
Total Src
Phosphorylation of ICAM-1 (pY512 ICAM-1)
Total ICAM-1
Phosphorylation of caveolin-1 (pY14 caveolin-1)
Total caveolin-1

control variables

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH)
Tumor necrosis factor receptor 1 (TNF-R1)
β-actin

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

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