SD rats were divided into three groups, including the control (6 weeks), HF-LPLI/6-week and HF-LPLI/12-week groups, and were analyzed at the corresponding time point. Rats were anesthetized with 10% chloral hydrate and treated with fluorescein that was injected through the tail vein. At 60 min post-infection images were obtained after intraperitoneal injection of D-luciferin (150 mg/kg body weight; Thermo Fisher Scientific, Inc.), followed by 50 μl D-luciferin (0.75 mg in PBS) through the oral cavity. The IVIS Lumina image system (Xenogen Corporation, Alameda, CA, EUA) was used to capture the images (44 (link)).
Protocol detail
In Vivo Bioluminescence Imaging of Rats
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Body weight
Chloral hydrate
Fluorescein
Infection
Intraperitoneal injection
Luciferin
Oral cavity
Rats
Tail
Vein
Corresponding Organization :
Other organizations : Liaocheng People's Hospital
Top 5 similar protocols
Variable analysis
independent variables
- Group: Control (6 weeks), HF-LPLI/6-week, HF-LPLI/12-week
- Outcomes at the corresponding time point
- Anesthesia with 10% chloral hydrate
- Fluorescein injection through the tail vein
- D-luciferin injection (150 mg/kg body weight) intraperitoneally, followed by 50 μl D-luciferin (0.75 mg in PBS) through the oral cavity
- IVIS Lumina image system used to capture the images
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!