Antibodies for TRIM67 and TRIM9 Characterization

Antibodies: TRIM67 rabbit polyclonal generated using murine TRIM67 recombinant protein aa45-73 [used at 1:1000 for immunoblot, 1:500 for immunohistochemistry (IHC)]; TRIM9 rabbit polyclonal antibody (used at 1:1000 for immunoblot; Winkle et al., 2014 (link)); mouse monoclonal against GAPDH [sc-166545, Santa Cruz Biotechnology (SCBT), used at 1:2500]; mouse monoclonal against myc tag (sc-40, SCBT, used at 1:1000); mouse monoclonal against HA tag (05-904, Millipore, used at 1:1000); and mouse monoclonal against β-III-tubulin (801202, BioLegend, used at 1:2000 for immunoblot, 1:1000 for IHC). Fluorescent secondary antibodies labeled with Alexa Fluor 568 or Alexa Fluor 647 were obtained from Invitrogen.
The plasmid encoding myc-tagged human TRIM9 aa139-781 (myc-TRIM9ΔRING) was described previously (Winkle et al., 2014 (link)). The plasmid encoding myc-tagged mouse TRIM67 aa158-783 (myc-TRIM67ΔRING) was generated by cloning the partial mouse TRIM67 sequence into the pcs2 vector. pcDNA3-DCC-HA (HA-DCC) was a generous gift from Dr. Marc Tessier Lavigne (Rockefeller).

Free full text: Click here

Boyer N.P., Monkiewicz C., Menon S., Moy S.S, & Gupton S.L. (2018). Mammalian TRIM67 Functions in Brain Development and Behavior. eNeuro, 5(3), ENEURO.0186-18.2018.

Publication 2018

sc-166545 Alexa Fluor 568 Alexa Fluor 647

Alexa 568 Alexa fluor 647 Antibodies Antibody Fluorescent antibodies Gapdh Human Immunoblot Immunohistochemistry Marc Mouse Murine Plasmid Rabbit Recombinant protein Tubulin Vector

Corresponding Organization :

Other organizations : University of North Carolina at Chapel Hill

Top 5 similar protocols

Variable analysis

independent variables

Antibody used: TRIM67 rabbit polyclonal, TRIM9 rabbit polyclonal, mouse monoclonal against GAPDH, mouse monoclonal against myc tag, mouse monoclonal against HA tag, mouse monoclonal against β-III-tubulin
Plasmid encoding: myc-tagged human TRIM9 aa139-781 (myc-TRIM9ΔRING), myc-tagged mouse TRIM67 aa158-783 (myc-TRIM67ΔRING), pcDNA3-DCC-HA (HA-DCC)

dependent variables

Not explicitly mentioned

control variables

Antibody dilutions used for immunoblot and immunohistochemistry (IHC)
Fluorescent secondary antibodies labeled with Alexa Fluor 568 or Alexa Fluor 647

positive controls

Not specified

negative controls

Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!