Characterization of Metastatic Brain Tumors

Using the operating microscope (Leica A60), metastatic tumors in the brain were isolated from the tumor-bearing mice. Brain tissues isolated from normal mice were used as controls. Activity of NE in tumor and normal brain tissues were measured by using a SensoLyte® Green Elastase Assay Kit (#AS-72178, AnaSpec, CA). For characterization of CXCR4, Western Blot analysis was performed according to the standard procedures as previously described ^{[25 (link)]}. Antibodies used in this study included anti-CXCR4 (#NB100–74396, Novus), HRP anti-rabbit IgG (#656120, invitrogen), beta-actin (c643802, BioLegend), and HRP anti-mouse IgG (AP307P, Sigma-Aldrich).

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Zhang S., Deng G., Liu F., Peng B., Bao Y., Du F., Chen A.T., Liu J., Chen Z., Ma J., Tang X., Chen Q, & Zhou J. (2020). Autocatalytic Delivery of Brain Tumor-targeting, Size-shrinkable Nanoparticles for Treatment of Breast Cancer Brain Metastases. Advanced functional materials, 30(14), 1910651.

Publication 2020

SensoLyte® Green Elastase Assay Kit NB100–74396 HRP anti-rabbit IgG beta-actin HRP anti-mouse IgG

Anti igg Antibodies Assay Beta actin Brain Brain metastatic tumors Cxcr4 Elastase Microscope Mouse Normal tissues Novus Rabbit Tumor Western blot analysis

Corresponding Organization : Renmin Hospital of Wuhan University

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Variable analysis

independent variables

Tumor isolation from tumor-bearing mice
Brain tissue isolation from normal mice

dependent variables

Activity of NE (Neutrophil Elastase) in tumor and normal brain tissues
Characterization of CXCR4 via Western Blot analysis

control variables

Brain tissues isolated from normal mice used as controls for NE activity measurement
Standard procedures for Western Blot analysis as previously described [25]

controls

Positive control: Not explicitly mentioned.
Negative control: Brain tissues isolated from normal mice for NE activity measurement.

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