Spectral-domain (SD)-OCT was used to noninvasively analyze retinal neuronal structure.21 (link) Mice were anesthetized with an intraperitoneal injection of a mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg). Pupils were dilated with tropicamide and phenylephrine, and mice were then positioned on the imaging platform of the Spectral-Domain Ophthalmic Imaging System (Envisu R2200; Bioptigen Inc., Morrisville, NC, USA). An en face scan consisting of 100 B-scans in a rectangular pattern was performed, in which high-resolution B-scans consist of 1000 A-scans with 1.4-μm resolution and 1.4-mm width. Retinal thickness within a donut-shaped area centered at the optic nerve disc was measured in an annular pattern. The inner and outer radiuses of the donut-shaped area were 200 and 700 μm from the center of the optic nerve disc, respectively. Retinal thickness maps and statistics were obtained using Bioptigen's report generator. The analysis included the “mouse report” template, which uses techniques for automated segmentation of the retinal images to generate depth maps and statistical tables of each retinal layer at various depths. The ganglion cell complex (GCC) is defined as the three innermost retinal layers: the nerve fiber layer, the GCL, and the inner plexiform layer.
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