Normal human gastric mucosa epithelial cells and gastric cancer cells were transfected with pcDNA3.1-RMRP, empty vector, si-RMRP or si-NC using Lipofectamine 2000 reagent and Opti-MEM I Reduced Serum Medium (Life Technologies) in accordance with the manufacturer's instructions, respectively. Cell proliferation was analyzed with a real-time cell analyzer (RTCA; ACEA Biosciences, San Diego, CA) as previously reported [16 (link)].
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