Inhibitor-based Mitochondrial Respiration Assay

PTP Inhibitor XXII (CAS 765317-72-4)38 (link) was purchased from EMD Millipore. IOXI (CAS 5852-78-8) was purchased from Cayman Chemicals. Oligomycin A, FCCP (trifluorocarbonylcyanide phenylhydrazone), rotenone, and DMOG (Dimethyloxaloglycine) were purchased from Tocris Bioscience. Antimycin A was purchased from Enzo Life Sciences.
Rabbit polyclonal anti-RNF213 antibodies were generated and used as described39 (link). Other antibodies were purchased and used for immunoprecipitations and/or immunoblotting at manufacturer-recommended concentrations (Supplementary Table 5).

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Banh R.S., Iorio C., Marcotte R., Xu Y., Cojocari D., Rahman A.A., Pawling J., Zhang W., Sinha A., Rose C.M., Isasa M., Zhang S., Wu R., Virtanen C., Hitomi T., Habu T., Sidhu S.S., Koizumi A., Wilkins S.E., Kislinger T., Gygi S.P., Schofield C.J., Dennis J.W., Wouters B.G, & Neel B.G. (2016). PTP1B regulates non-mitochondrial oxygen consumption via RNF213 to promote tumour survival during hypoxia. Nature cell biology, 18(7), 803-813.

Publication 2016

PTP Inhibitor XXII Oligomycin A FCCP rotenone Antimycin A

Anti antibodies Antibodies Antimycin a Cayman Fccp Immunoprecipitations Oligomycin Phenylhydrazone Rabbit Rnf213 Rotenone

Corresponding Organization :

Other organizations : University of Toronto, Princess Margaret Cancer Centre, University Health Network, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Harvard University, NYU Langone Health, Kyoto University, Oxford Research Group, University of Oxford

Top 5 similar protocols

Variable analysis

independent variables

PTP Inhibitor XXII (CAS 765317-72-4)
IOXI (CAS 5852-78-8)
Oligomycin A
FCCP (trifluorocarbonylcyanide phenylhydrazone)
Rotenone
DMOG (Dimethyloxaloglycine)
Antimycin A

dependent variables

Immunoprecipitations and/or immunoblotting

control variables

Rabbit polyclonal anti-RNF213 antibodies

controls

Positive control: Rabbit polyclonal anti-RNF213 antibodies
Negative controls: Not explicitly mentioned

Annotations

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