Two-Photon Laser Scanning Microscopy Acquisition

For 2P-LSM acquisition, the open-source MATLAB-based software application ScanImage^® (Vidrio Technologies, Ashburn, VA, United States) (Pologruto et al., 2003 (link)) was used. The custom-made MATLAB-based software MSparkles, GraphPad Prism 8 and Microsoft Office Excel 2016 were used for data analysis. Immunohistochemical data were visualized and modified using the ZEN blue imaging software (Zeiss, Oberkochen, Germany) and the ImageJ collection Fiji. For figure layout, the Adobe Creative Suite 2021 was used (Adobe InDesign^®, Adobe Illustrator^®, Adobe Photoshop^®).

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Rieder P., Gobbo D., Stopper G., Welle A., Damo E., Kirchhoff F, & Scheller A. (2022). Astrocytes and Microglia Exhibit Cell-Specific Ca2+ Signaling Dynamics in the Murine Spinal Cord. Frontiers in Molecular Neuroscience, 15, 840948.

Publication 2022

ZEN blue imaging software

Prism

Corresponding Organization : Saarland University

Other organizations : Heidelberg University, University Hospital Heidelberg

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