Live Imaging of Drosophila Embryos

Raskol-YFP [55 (link)] homozygous female flies were crossed to DE-cad-mCherry [49 (link)] homozygous males. Embryos were collected overnight on grape juice agar plates and transferred to microscope slides coated with double-sided tape. Embryos were manually dechorionated and immediately transferred to halocarbon oil on coverslips with the dorsal side facing down. Coverslips were then attached to imaging chambers using double sided tape and imaged on a Nikon A1 scanning confocal microscope.

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Raza Q., Choi J.Y., Li Y., O’Dowd R.M., Watkins S.C., Chikina M., Hong Y., Clark N.L, & Kwiatkowski A.V. (2019). Evolutionary rate covariation analysis of E-cadherin identifies Raskol as a regulator of cell adhesion and actin dynamics in Drosophila. PLoS Genetics, 15(2), e1007720.

Publication 2019

Nikon A1 scanning confocal microscope

Agar Confocal microscope Embryos Female Flies Grape Homozygous Males Microscope

Corresponding Organization : New York University

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Variable analysis

independent variables

Genotype of flies: Raskol-YFP homozygous females crossed with DE-cad-mCherry homozygous males

dependent variables

Not explicitly mentioned

control variables

Embryo collection: Overnight collection on grape juice agar plates
Embryo preparation: Manual dechorionation and immediate transfer to halocarbon oil on coverslips with dorsal side facing down
Imaging: Nikon A1 scanning confocal microscope

controls

Positive control: Not mentioned
Negative control: Not mentioned

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