Trichomoniasis Diagnosis Protocol

The Ethics and Research Committees at Hospital General “Dr. Manuel Gea Gonzalez” (HGMGG) approved this study, with reference number 12-75-2015. Recovered samples, used primarily for pathogen diagnosis of 28 women with trichomoniasis who attended the gynecology service of the HGMGG between 2015 and 2019, were analyzed [20 (link)]; thus, vaginal swab samples confirmed by microscopy with T. vaginalis were placed in sterile plastic tubes with 0.9% physiological saline solution and kept at −70 °C until DNA isolation.

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Martinez-Hernandez F., Sanchez-Aguillon F., Martinez-Ocaña J., Gonzalez-Arenas N.R., Romero-Valdovinos M., Lopez-Escamilla E., Maravilla P, & Villalobos G. (2023). Genetic Variability of the Internal Transcribed Spacer and Pyruvate:Ferredoxin Oxidoreductase Partial Gene of Trichomonas vaginalis from Female Patients. Microorganisms, 11(9), 2240.

Publication 2023

0 9 saline Diagnosis Isolation Microscopy Pathogen Physiological Sterile Trichomoniasis Vaginal Women

Corresponding Organization : Universidad Autónoma Metropolitana

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Presence or absence of Trichomonas vaginalis based on microscopy analysis

control variables

Vaginal swab samples were collected from 28 women with trichomoniasis who attended the gynecology service of the HGMGG between 2015 and 2019
Vaginal swab samples were confirmed by microscopy to contain T. vaginalis
Vaginal swab samples were placed in sterile plastic tubes with 0.9% physiological saline solution and kept at −70 °C until DNA isolation

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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