Antibody Profiling for Synaptic and Immune Markers

The following primary antibodies were used for this study: mouse monoclonal anti synaptophysin (SP) (clone SVP38, 1.4 ug/ml, Sigma), guinea pig polyclonal anti VGlut11 (1:1000, Millipore), rabbit polyclonal anti postsynaptic density 95 (PSD95, 1 ug/ml, Invitrogen), rat monoclonal anti CD68 (clone FA-11, 1.4 ug/ml, Bio-Rad), rabbit polyclonal anti Iba-1 (1:1000, Wako), rat monoclonal anti C3 (clone 11H9, 0.5ug/ml, Abcam) and rabbit monoclonal anti C1q (clone 27.1, hybridoma supernatant previously described (41 (link), 42 (link))). The specificity of the anti-mouse C1q antibody was demonstrated by the lack of C1q staining detected in the hippocampal CA1-SR of an Arctic C1q conditional KO (ArcC1qa^FL/FL :Cx3cr1^ERT2) compared with the Arc C1qa^FL/FL at super-resolution level (Supp Figure 2) and characterized previously (41 (link)).

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Gomez-Arboledas A., Fonseca M.I., Kramar E., Chu S.H., Schartz N., Selvan P., Wood M.A, & Tenner A.J. (2023). C5aR1 signaling promotes region and age dependent synaptic pruning in models of Alzheimer’s Disease. bioRxiv.

Publication Preprint 2023

SP) (clone SVP38 PSD95 rat monoclonal anti C3 (clone 11H9,

Anti synaptophysin Antibodies Antibody specificity Clone Guinea pig Hybridoma Mouse Postsynaptic density Rabbit

Corresponding Organization : University of California, Irvine

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Variable analysis

independent variables

Mouse monoclonal anti synaptophysin (SP) (clone SVP38, 1.4 ug/ml, Sigma)
Guinea pig polyclonal anti VGlut11 (1:1000, Millipore)
Rabbit polyclonal anti postsynaptic density 95 (PSD95, 1 ug/ml, Invitrogen)
Rat monoclonal anti CD68 (clone FA-11, 1.4 ug/ml, Bio-Rad)
Rabbit polyclonal anti Iba-1 (1:1000, Wako)
Rat monoclonal anti C3 (clone 11H9, 0.5ug/ml, Abcam)
Rabbit monoclonal anti C1q (clone 27.1, hybridoma supernatant previously described (41 (link), 42 (link)))

dependent variables

Not explicitly mentioned

control variables

Arc C1qa^FL/FL : Cx3cr1^ERT2 (Arctic C1q conditional KO)

controls

Positive control: Arc C1qa^FL/FL (no C1q KO)
Negative control: Arc C1qa^FL/FL : Cx3cr1^ERT2 (Arctic C1q conditional KO)

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