Protocol detail

Diagnostic Techniques for Intestinal Parasites

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The method was evaluated using DNA from 143 stool samples submitted to the Institute of Specific Prophylaxis and Tropical Medicine at the Medical University of Vienna between 2011 and 2018 for diagnostic analysis. The standard diagnostic test of the study samples at our institute included microscopic analysis of stool for all intestinal parasites, immunoassay analysis (Antigens) for G. intestinalis and E. histolytica, and FRET-based qPCR (DNA) for Blastocystis spp., C. parvum, C. hominis, E. histolytica, E. dispar and G. intestinalis. All samples were anonymized. The Qiagen kit for stool DNA extraction (Qiagen GmbH, Vienna, Austria) was used for the samples’ DNA extraction according to manufacturer instructions.

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Lamien-Meda A., Schneider R., Walochnik J., Auer H., Wiedermann U, & Leitsch D. (2020). A novel 5-Plex qPCR-HRM assay detecting human diarrheal parasites. Gut Pathogens, 12, 27.

Publication 2020

Antigens Blastocystis C parvum Diagnostic Diagnostic test Fret Immunoassay Intestinal parasites Microscopic Stool

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Protocol cited in 2 other protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Detection of intestinal parasites (microscopic analysis)
Detection of Giardia intestinalis and Entamoeba histolytica (immunoassay analysis)
Detection of Blastocystis spp., Cryptosporidium parvum, Cryptosporidium hominis, Entamoeba histolytica, Entamoeba dispar, and Giardia intestinalis (FRET-based qPCR)

control variables

Stool samples were submitted to the Institute of Specific Prophylaxis and Tropical Medicine at the Medical University of Vienna between 2011 and 2018 for diagnostic analysis
All samples were anonymized
Qiagen kit for stool DNA extraction was used according to manufacturer instructions

controls

No positive or negative controls were explicitly mentioned in the provided information.

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