Transcriptomic Analysis of m6A Pathway in Poplar

Total RNA of leaves, roots, xylem and phloem from Poplar 84K and LBD15-oe plants were isolated by RNA Easy Fast Plant Tissue Kit (TIANGEN, Beijing, China). The cDNA was generated by reverse transcription using FastKing RT Kit (TIANGEN, Beijing, China). The qRT-PCR analysis of m⁶A pathway genes were performed with SYBR^® rapid quantitative PCR Kit (KAPA KK4601, Pleasanton, CA, USA) using the methods described previously [35 ]. Pagactin was used as a reference [24 (link)]. The primers of all the genes were listed in Table S5, and the results were analyzed using the 2^−ΔΔCt method [24 (link)].

Free full text: Click here

Sun X., Wu W., Yang Y., Wilson I., Shao F, & Qiu D. (2022). Genome-Wide Identification of m6A Writers, Erasers and Readers in Poplar 84K. Genes, 13(6), 1018.

Publication 2022

RNA Easy Fast Plant Tissue Kit FastKing RT Kit SYBR® rapid quantitative PCR Kit

Cdna Genes Phloem Plant rna Plants Poplar Primers Reverse transcription Roots Tissue Xylem

Corresponding Organization : Chinese Academy of Forestry

Other organizations : Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food

Top 5 similar protocols

Variable analysis

independent variables

Poplar 84K
LBD15-oe

dependent variables

Expression of m6A pathway genes

control variables

Pagactin (reference gene)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!