Histopathological Analysis of Cedar Wood Nanoencapsulated Anopheles Larva

To study the mode of action of cedar wood nanoencapsulated on larva, the treated dead larva of Anopheles was studied through histopathology. The mortal larvae was removed from the treated solution and stored in buffered formalin reagent (pH 7.2). The larva was dehydrated by passing through graded ethanol series and embedded in paraffin wax^{67 (link)}. A thin longitudinal section (LS) of the larva stained with Hematoxylin and Eosin (Hi-Media labs) was cut using microtome (Leica, Germany) and mounted on glass slide. The LS of the midgut region were examined under an Upright Olympus microscope. A setup containing tween, span and water served as control was also performed for reference.