Histological Analysis of Ram Muscle Fibers

A total of 32 rams were randomly selected from the population of 76 to conduct histological detection at Chengdu Lilai Biotechnology Co., Ltd. The methods were referenced by Cui et al. (31 (link)), with some modifications. For haematoxylin and eosin staining (HE staining), longissimus dorsi samples were fixed overnight in 10% neutral formaldehyde solution, embedded in paraffin, and then cut into 5 μm sections. The sections were further stained according to a standard protocol and examined with a BA210Digital light microscope (Motic China Group Co., Ltd., Xiamen, China). The fiber number in each sample was manually counted in three different fields (400 × mirror), and the average was calculated. Muscle fiber diameter and perimysium thickness were measured with Motic Images Advanced 3.2 software.

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Zhang X., Liu C., Kong Y., Li F, & Yue X. (2022). Effects of intramuscular fat on meat quality and its regulation mechanism in Tan sheep. Frontiers in Nutrition, 9, 908355.

Publication 2022

Motic Images Advanced 3.2 software

Embedded paraffin Eosin Fiber Formaldehyde solution Haematoxylin Microscope light Muscle Rams

Corresponding Organization : Ministry of Agriculture and Rural Affairs

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Variable analysis

independent variables

Selection of 32 rams from a population of 76

dependent variables

Histological detection
Fiber number
Muscle fiber diameter
Perimysium thickness

control variables

Fixation of longissimus dorsi samples in 10% neutral formaldehyde solution
Embedding in paraffin
Cutting sections into 5 μm thickness
Staining protocol
Examination with BA210Digital light microscope

positive controls

None specified

negative controls

None specified

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