RIP was implemented according to the procedures of Magna RIP™ Kit (Millipore, MA, USA). Cell lysates were separately incubated with Ago2 antibody and IgG antibody and added with agarose beads (Bio-Rad, CA, USA). circNINL, miR-3918 and FGFR1 expression was detected by RT-qPCR [22 (link)].
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