Protein Solubilization and Immunoblotting

Protein solubilization and immunoblotting were conducted as previously described [15 (link)]. The following commercially available antibodies were used: anti-TAZ (V386) (#4883; Cell Signaling Technology, Beverly, MA, USA), anti-survivin (71G4B7) (#2808; Cell Signaling Technology), anti-p53 (sc-126; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p21 (F-5, Santa Cruz Biotechnology), and anti-β-actin-HRP (C4; Santa Cruz Biotechnology).

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Miyajima C., Hayakawa Y., Inoue Y., Nagasaka M, & Hayashi H. (2022). HMG-CoA Reductase Inhibitor Statins Activate the Transcriptional Activity of p53 by Regulating the Expression of TAZ. Pharmaceuticals, 15(8), 1015.

Publication 2022

anti-TAZ (V386) anti-survivin (71G4B7) anti-p53 (sc-126; anti-p21 (F-5, anti-β-actin-HRP (C4;

Actin Antibodies Protein Survivin

Corresponding Organization : Nagoya City University

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Variable analysis

independent variables

Protein solubilization and immunoblotting techniques

dependent variables

Protein expression levels of TAZ, survivin, p53, and p21

control variables

β-actin expression (used as a loading control)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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