Quantifying Caspase-3 Activation in FFPE Tumors

Single-color IHC was performed on FFPE tumor slices after six days of treatment as previously described using cleaved Caspase-3 (cC3) primary antibody (Cell Signaling Technologies, Danvers, MA, 1:200)^{35 (link)}. Whole slide imaging and digital archiving was performed using the Nanozoomer Digital Pathology system (Hamamatsu, Shizuoka, Japan), and 20 × fields (7–13 fields per group) were automatically counted using QuPath or manually counted using Fiji ImageJ Cell Counter^{36 (link)}.

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Daniel S.K., Sullivan K.M., Dickerson L.K., van den Bijgaart R.J., Utria A.F., Labadie K.P., Kenerson H.L., Jiang X., Smythe K.S., Campbell J.S., Pierce R.H., Kim T.S., Riehle K.J., Yeung R.S., Carter J.A., Barry K.C, & Pillarisetty V.G. (2024). Reversing immunosuppression in the tumor microenvironment of fibrolamellar carcinoma via PD-1 and IL-10 blockade. Scientific Reports, 14, 5109.

Publication 2024

cC3) primary antibody Nanozoomer Digital Pathology system

Corresponding Organization :

Other organizations : University of Washington, Fred Hutch Cancer Center

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Variable analysis

independent variables

Treatment duration (6 days)

dependent variables

Cleaved Caspase-3 (cC3) expression

control variables

FFPE tumor slices

controls

None specified
None specified

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