Isolation and Characterization of Retinal Monocytes

Cells were isolated from the retina and choroid as described previously [62 (link)]. The samples were analyzed using FACSAria III (BD Biosciences, San Jose, CA, USA) and FlowJo software (Tree Star, Ashland, OR, USA). Anti-F4/80-APC, anti-Ly6C-FITC, anti-Ly6G-APC-Cy7 (all from BioLegend, San Diego, CA, USA), and anti-CD11b-PE (eBioscience, San Diego, CA, USA) were used. Monocytes subsets were identified as CD11bhiF4/80hiLy6Chi/int/lo and can be subdivided into 3 subsets; classical, intermediate, and non-classical monocytes [63 (link)].

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Ahamed W., Yu R.M., Pan Y., Iwata T., Barathi V.A., Wey Y.S., Tun S.B., Qiu B., Tan A., Wang X., Cheung C.M., Wong T.Y, & Yanagi Y. (2022). HTRA1 Regulates Subclinical Inflammation and Activates Proangiogenic Response in the Retina and Choroid. International Journal of Molecular Sciences, 23(18), 10206.

Publication 2022

FACSAria III Anti-F4/80-APC anti-Ly6C-FITC anti-Ly6G-APC-Cy7 anti-CD11b-PE

Cd11b Cells Choroid Fitc Monocytes Retina Tree

Corresponding Organization :

Other organizations : Singapore National Eye Center, Singapore Eye Research Institute, Tokyo Medical Center, National Hospital Organization, Duke-NUS Medical School, National University of Singapore, Agency for Science, Technology and Research, Institute of Molecular and Cell Biology

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Variable analysis

independent variables

Anti-F4/80-APC
Anti-Ly6C-FITC
Anti-Ly6G-APC-Cy7
Anti-CD11b-PE

dependent variables

Monocyte subsets (classical, intermediate, and non-classical)

control variables

Cells isolated from the retina and choroid as described previously [62]

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