Protocol detail

Dexamethasone Effects on ACTH and Corticosterone

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In Cohort 4 mice were treated with dexamethasone (0.1 mg/kg ip, Oradexon, Organon, Oss, Hollandia) during the early morning (at the time of the beginning of the dark phase or active period of the animals, with the highest ACTH-corticosterone level) [53 (link)]. Trunk blood was collected 6 h after ip injections in ice-cooled Eppendorf tubes. Serum was separated by centrifugation (3000 rpm for 10 min at 4 °C) and stored at −20 °C until assayed. The ACTH and corticosterone concentrations were determined by radioimmunoassay in duplicates as described earlier [54 (link)].

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Várkonyi D., Török B., Sipos E., Fazekas C.L., Bánrévi K., Correia P., Chaves T., Farkas S., Szabó A., Martínez-Bellver S., Hangya B, & Zelena D. (2022). Investigation of Anxiety- and Depressive-like Symptoms in 4- and 8-Month-Old Male Triple Transgenic Mouse Models of Alzheimer’s Disease. International Journal of Molecular Sciences, 23(18), 10816.

Publication 2022

dexamethasone

Animals Blood Centrifugation Corticosterone Dexamethasone Ip injections Mice Oradexon Radioimmunoassay Serum

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Variable analysis

independent variables

Dexamethasone (0.1 mg/kg ip, Oradexon, Organon, Oss, Hollandia)

dependent variables

ACTH concentration
Corticosterone concentration

control variables

Time of dexamethasone injection (during the early morning, at the time of the beginning of the dark phase or active period of the animals, with the highest ACTH-corticosterone level)
Time of trunk blood collection (6 h after ip injections)
Blood sample processing (centrifugation at 3000 rpm for 10 min at 4 °C)
Sample storage (-20 °C until assayed)

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