CDH4 Modulation by siRNA and Overexpression

siRNA oligonucleotides targeting CDH4, along with negative control siRNA, were procured from GenePharma (Shanghai, China). Lentiviral constructs that express the full-length CDH4 or shRNA sequences that targeted CDH4 were generated by cloning the corresponding sequences into the pCDH-CMV-MCS-EF1-Puro vector. The HA-Ub plasmid (pCMV-HA-Ub), His-CDH4 plasmid (pCMV-6 × His-CDH4), and Flag-β-catenin plasmid (pCDEF-FLAG-β-catenin) were obtained from Miaoling (Wuhan, China). Transfection techniques adhered to previously reported methods and manufacturer's instructions [18 (link)]. The specific sequences of the siRNA or shRNA mentioned above can be found in Additional file 1: Table S1.

Free full text: Click here

Wu L., Xiao J., Yi D., Ding H., Wang R., Duan Z., Liu Z., Shi X., Shen M, & Sang J. (2024). Cytosolic Cadherin 4 promotes angiogenesis and metastasis in papillary thyroid cancer by suppressing the ubiquitination/degradation of β-catenin. Journal of Translational Medicine, 22, 201.

Publication 2024

siRNA oligonucleotides targeting

Corresponding Organization :

Other organizations : Nanjing Drum Tower Hospital, Nanjing Medical University, Jiangsu Province Hospital

Top 5 similar protocols

Variable analysis

independent variables

SiRNA oligonucleotides targeting CDH4
ShRNA sequences that targeted CDH4
Lentiviral constructs that express the full-length CDH4

dependent variables

Not explicitly mentioned

control variables

Negative control siRNA

controls

Negative control siRNA

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!