Adenosine 5’-triphosphate (ATP) assays were performed to assess cell proliferation, which correlates with cell viability in monolayer cultures of osteogenically differentiated hMSCs and chondrocytes. Cell viability was measured after 10 min, 24 h and 48 h of treatment with various concentrations (no TXA, 10 mg/mL, 20 mg/mL and 50 mg/mL) of TXA using the CellTiter-Glo® luminescent cell viability assay (Promega, Madison, WI, USA), as previously described [22 (link)].
After TXA-treatment, hMSCs and chondrocytes that were used for biochemical investigations were trypsinated and seeded in new 96-well-plates (Greiner Bio-One GmbH) at a density of 3 x 103 cells per cm2.
ATP assays were performed after 10 min, 24 h and 48 h. According to the user’s guide, the cells were mixed with 100 μL of CellTiter-Glo® (Promega GmbH, Mannheim, Germany) reagent, a composition of CellTiter-Glo® substrate with CellTiter-Glo® buffer. Cells were incubated in this reagent for 10 min before luminescence was measured using a plate-reading luminometer (Promega GmbH).
After TXA-treatment, hMSCs and chondrocytes that were used for biochemical investigations were trypsinated and seeded in new 96-well-plates (Greiner Bio-One GmbH) at a density of 3 x 103 cells per cm2.
ATP assays were performed after 10 min, 24 h and 48 h. According to the user’s guide, the cells were mixed with 100 μL of CellTiter-Glo® (Promega GmbH, Mannheim, Germany) reagent, a composition of CellTiter-Glo® substrate with CellTiter-Glo® buffer. Cells were incubated in this reagent for 10 min before luminescence was measured using a plate-reading luminometer (Promega GmbH).
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