Measuring Cellular Glucose Uptake

Cells seeded in 6-well plates were treated with or without 1 μM gefitinib or erlotinib. Cell were washed with PBS three times and then incubated with glucose-free medium for 4 h. After wash with PBS for three times, the cells were incubated with PBS containing 2-NBDG (100 μM; Sigma-Aldrich) for 20 min at 37 °C and then subjected to flow cytometry analysis (BD Biosciences) or microscope image processing (ThermoFisher).

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Cheng F.J., Chen C.H., Tsai W.C., Wang B.W., Yu M.C., Hsia T.C., Wei Y.L., Hsiao Y.C., Hu D.W., Ho C.Y., Li T.S., Wu C.Y., Chou W.Y., Yu Y.L., Tang C.H., Chen C.Y., Chen C.M., Hsu J.L., Chen H.F., Chen Y., Tu C.Y., Hung M.C, & Huang W.C. (2020). Cigarette smoke-induced LKB1/AMPK pathway deficiency reduces EGFR TKI sensitivity in NSCLC. Oncogene, 40(6), 1162-1175.

Publication 2020

2-NBDG

2 nbdg Cells Erlotinib Flow cytometry Gefitinib Glucose Microscope

Corresponding Organization :

Other organizations : China Medical University, China Medical University Hospital, Chang Bing Show Chwan Memorial Hospital, National Yang Ming University Hospital, National Yang Ming Chiao Tung University, Chung Shan Medical University, National Chung Hsing University

Top 5 similar protocols

Protocol cited in 5 other protocols

Variable analysis

independent variables

Treatment with or without 1 μM gefitinib or erlotinib

dependent variables

2-NBDG uptake (analyzed by flow cytometry or microscope image processing)

control variables

Cell seeding in 6-well plates
Washing cells with PBS three times
Incubation with glucose-free medium for 4 h
Washing cells with PBS three times
Incubation with PBS containing 2-NBDG (100 μM) for 20 min at 37 °C

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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