Fluorescent Lipid Probes and MTP Inhibitor Assay

PC (no. 131601C) and fluorescently labeled ammonium salts of PEs with the acyl groups, NBD-1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE; no. 810143C), NBD-POPE (no. 810118P), or NBD-DOPE (no. 810145C) were obtained from Avanti Polar Lipids (Alabaster, AL). NBD-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (DPPE; no. N360) was obtained from Invitrogen (Thermo Fisher Scientific). Lomitapide, an MTP inhibitor, was obtained from Sigma (no. SML1385-5MG). The Cos-7 and Huh-7 cell lines, and the plasmids pcDNA3 and pcDNA3-hMTP-FLAG, have been described previously (20 (link), 21 (link), 26 (link), 27 (link)). Anti-FLAG® M2 affinity agarose gel and other chemicals were purchased from Millipore Sigma (no. A2220-5ML, St. Louis, MO). Round bottom black 96-well assay plates were obtained from Costar (no. 3792; Kennebunk, ME). FLAG peptide was custom synthesized (GenScript).

Free full text: Click here

Anaganti N., Rajan S, & Hussain M.M. (2021). An improved assay to measure the phospholipid transfer activity of microsomal triglyceride transport protein. Journal of Lipid Research, 62, 100136.

Publication 2021

NBD-DOPE Lomitapide Anti-FLAG® M2 affinity agarose gel Round bottom black 96-well assay plates

Agarose Alabaster Ammonium salts Assay Cell lines Dmpe Dppe Flag peptide Hmtp Lipids Lomitapide Phosphoethanolamine Plasmids Pope Salt

Corresponding Organization : VA NY Harbor Healthcare System

Top 5 similar protocols

Variable analysis

independent variables

PC (no. 131601C)
NBD-1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE; no. 810143C)
NBD-POPE (no. 810118P)
NBD-DOPE (no. 810145C)
NBD-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (DPPE; no. N360)
Lomitapide, an MTP inhibitor

dependent variables

Not explicitly mentioned

control variables

Cos-7 and Huh-7 cell lines
PcDNA3 and pcDNA3-hMTP-FLAG plasmids
Anti-FLAG® M2 affinity agarose gel
Round bottom black 96-well assay plates
FLAG peptide

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!