Plasmid Transfection for Protein Expression

A PRL-1 (human protein tyrosine phosphatase type 4A, member 1 [PTP4A1]) plasmid was purchased (Origene, Rockville, MD, USA). The CMV6-AC vector containing the PTP4A1 gene was digested with the restriction enzymes Sgf1 and Mlu1 and included a GFP reporter gene and neomycin. A lentiviral vector including the PRL-1 gene was acquired from SeouLin Bioscience (Seongnam, Republic of Korea). A pLenti-RSV-EF1a vector containing PRL-1 was constructed as a tagged protein with C-terminal GFP and puromycin. To generate a control group, we used the AMAXA pCMV-GFP vector (Lonza, Basel, Switzerland) containing kanamycin, and maxGFP was digested with the restriction enzymes Kpn1 and Bgl2.