Bile Acid Metabolism and Liver Function Assay

Aspartate aminotransferase and alanine aminotransferase assay kits were purchased from Pointe Scientific (Canton. MI). GSK2330672 was purchased from MedChemExpress LLC (Monmouth Junction, NJ). Bile acid assay kit was purchased from Diazyme Laboratories (Poway, CA). AAV-FGF15 (under the control of an albumin promoter) was purchased from GeneCopoeia, Inc. (Rockville, MD). AAV-Null was purchased from Vector Biolabs Inc. (Malvern, PA). F4/80 antibody (Cat #. 70,076) was purchased from Cell Signaling Technology (Danvers, MA). Cytokeratin-19 antibody (ab52625) was purchased from Abcam (Waltham, MA). ZO-1 antibody (PA5-28858) was purchased from Thermo Fisher Scientific (Grand Island, NY).CDCA-d4 MaxSpec® Standard (CDCA-d4, No. 31366), tauro-conjugated CDCA-d4 MaxSpec® Standard (T-CDCA-d4, No. 31362), UDCA-d4 MaxSpec® Standard (UDCA-d4, No. 31368), and Tauro-conjugated UDCA-d4 MaxSpec® Standard (T-UDCA-d4, No. 31564) were purchased from Caymen Chemical Company (Ann Arbor, MI). FITC-dextran (MW:3000–5000) was purchased from Sigma Aldrich (St. Louis, MO).