Kidney sections (4 μm thick) were stained using standard techniques as previously described (Chodavarapu et al. 2013 (link)). Slides were incubated either with primary polyclonal goat anti-ACE2 (1:150, R&D, MN, USA) or rabbit anti-ADAM17 (1:100, Enzo, USA) overnight at 4°C. Washings were repeated and the sections were incubated with biotinylated donkey anti-goat or anti-rabbit IgG secondary antibody conjugated with Cyanine 3 fluorescent dye. MetaMorph software (Molecular Devices, CA, USA) was used for quantitation.
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Immunohistochemical Analysis of ACE2 and ADAM17
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Variable analysis
independent variables
- Primary antibody used: polyclonal goat anti-ACE2 or rabbit anti-ADAM17
- Quantitation of ACE2 or ADAM17 expression using MetaMorph software
- Kidney sections (4 μm thick)
- Incubation time: overnight at 4°C
- Secondary antibody: biotinylated donkey anti-goat or anti-rabbit IgG secondary antibody conjugated with Cyanine 3 fluorescent dye
- No positive or negative controls were explicitly mentioned in the provided information.
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