Micro-CT Analysis of In Vivo Implants

Eight-week in vivo implants were fixed in formalin and stored in PBS. Micro-computed tomography (micro-CT) data were acquired from the implants using a phoenix nanotom m scanner (General Electric) with 0.5-mm aluminum filtered x-rays (applied voltage 70 kV; current 260 mA). Transmission images were acquired during a 360° scan rotation with an incremental rotation step size of 0.25°. Reconstruction was performed using a modified Feldkamp algorithm at an isotropic voxel size of 25 µm. Threshold-based segmentation and 3D measurement analyses (bone mineral density and volume) were performed using ImageJ software^{54 (link)} with the BoneJ^{55 (link)} and 3D Shape^{56 (link)} extensions. For quantification of bone mineral density and volume, all data were normalized to the size of each construct. Three-dimensional rendering of the structures was performed using VGStudio MAX 2.2 software (Volume Graphics).

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Guerrero J., Dasen B., Frismantiene A., Pigeot S., Ismail T., Schaefer D.J., Philippova M., Resink T.J., Martin I, & Scherberich A. (2022). T-cadherin Expressing Cells in the Stromal Vascular Fraction of Human Adipose Tissue: Role in Osteogenesis and Angiogenesis. Stem Cells Translational Medicine, 11(2), 213-229.

Publication 2022

phoenix nanotom m scanner VGStudio MAX 2.2

Aluminum Bone mineral density Computed tomography Electric Formalin Reconstruction Scan Transmission X rays

Corresponding Organization : University of Basel

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Variable analysis

independent variables

Duration of in vivo implantation (8 weeks)

dependent variables

Bone mineral density
Bone volume

control variables

Formalin fixation of implants
Storage of implants in PBS
Micro-CT scan parameters (0.5-mm aluminum filtered x-rays, applied voltage 70 kV, current 260 mA, 360° scan rotation with 0.25° incremental step size)
Reconstruction using modified Feldkamp algorithm at 25 µm isotropic voxel size
Threshold-based segmentation and 3D measurement analyses using ImageJ software with BoneJ and 3D Shape extensions

controls

No positive or negative controls were explicitly mentioned in the provided information.

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