The molecular weight of the unprocessed PLGA and printed PLGA was determined by GPC using a Waters Alliance Separations Module e2695, Waters 2414 Refractive Index Detector, and Waters HSPgel columns in series (HR MB-L and HR 3.0 columns, 6.0 mm ID × 15 cm) (Waters, Milford, MA) [18 (link)]. For printed PLGA, scaffold was fabricated using conditions summarized in table 2 , then a small piece of the printed scaffold was cut and dissolved in tetrahydrofuran for the test. Polystyrene standards were used to report the relative molecular weights. The standard curve was generated from a 10-point calibration using Agilent EasiCal polystyrene standards. The data analysis was performed using Waters Empower 3 Chromatography Data software. Three samples of each composition were performed for statistical analysis.
Protocol detail
Molecular Weight Analysis of PLGA
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Corresponding Organization : University of Maryland, College Park
Other organizations : Cornell University, Rice University
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Variable analysis
independent variables
- Fabrication conditions summarized in table 2
- Molecular weight of unprocessed PLGA
- Molecular weight of printed PLGA
- Use of polystyrene standards to report relative molecular weights
- Generation of standard curve from 10-point calibration using Agilent EasiCal polystyrene standards
- Data analysis performed using Waters Empower 3 Chromatography Data software
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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