1-(3-Aminopropyl)silatrane (APS)-functionalized mica was used as the AFM substrate for all experiments.27 (link) Briefly, freshly cleaved mica was incubated in 4 mL APS (167 μM) in a cuvette for 30 min and then rinsed with double-distilled water (ddH2O) thoroughly, as described in ref 26 (link). Ten microliters of the sample were deposited onto the APS mica for 2 min, rinsed with ddH2O, and dried with a gentle Argon gas flow. Images were acquired using tapping mode in the air on a MultiMode 8, Nanoscope V system (Bruker, Santa Barbara, CA) using TESPA probes (320 kHz nominal frequency and a 42 N/m spring constant) from the same vendor.
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Functionalized Mica for Atomic Force Microscopy
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Corresponding Organization : University of Nebraska Medical Center
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Variable analysis
independent variables
- Incubation time of mica in APS solution (30 min)
- Morphology of the sample deposited on APS-functionalized mica, as observed by atomic force microscopy (AFM)
- Concentration of APS solution (167 μM)
- Volume of sample deposited on APS mica (10 μL)
- Incubation time of sample on APS mica (2 min)
- Rinsing with double-distilled water (ddH2O)
- Drying method (gentle Argon gas flow)
- AFM imaging mode (tapping mode in air)
- AFM system (MultiMode 8, Nanoscope V, Bruker)
- AFM probe type (TESPA, 320 kHz nominal frequency, 42 N/m spring constant)
- Not specified
- Not specified
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