Quantifying Transplanted hADMSCs in Mice Livers

To quantify the transplanted hADMSCs that homed to the mice livers, a human Down syndrome sequence-based real-time PCR quantification system was used in the current study [7 (link)]. In brief, hepatic genomic DNA samples were extracted using a QIAamp genomic DNA extraction kit (Qiagen, Hilden, Germany). A pair of primers (5′- ATGCTGATGTCTGGGTAGGGTG-3′ and 5’-TGAGTCAGGAGCCAGCGTATG-3′) that generate a 141-bp fragment of human Down syndrome region at chromosome 21 was used to quantify the human-derived cells. To verify the quantitative PCR results, we performed an immunohistochemistry (IHC) assay using human albumin antibody in sectioned mice liver samples.