The following antibodies were used for immunofluorescence assays (IFA) and western blot (WB) analysis at the indicated dilutions: mouse anti-HA monoclonal antibody HA.11 (Covance; 1:500 WB); rabbit polyclonal anti-HA SG77 (ThermoFisher; 1:500 IFA and WB); mouse anti-Flag monoclonal antibody clone M2 (Sigma; 1:300 IFA); mouse anti-EXP1 monoclonal antibody (Lisewski et al., 2014 (link)) (1:500 WB); mouse anti-EXP2 monoclonal antibody clone 7.7 (Hall et al., 1983 (link)) (1:500 IFA and WB); rabbit polyclonal anti-SBP1 (Blisnick et al., 2000 (link)) (1:500 IFA); mouse anti-cMYC monoclonal antibody 9E10 (ThermoFisher; 1:166 IFA and WB); rabbit polyclonal anti-Plasmodium Aldolase ab207494 (Abcam; 1:500 WB); rabbit polyclonal anti-RFP R10367 (ThermoFisher; 1:500 WB).
Protocol detail
Antibody Characterization for Immunofluorescence and Western Blot
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Aldolase
Anti antibody
Antibodies
Assays immunofluorescence
Clone
Dilutions
Exp1
Immunofluorescence assays
Monoclonal antibody
Mouse
Plasmodium
Rabbit
Western blot
Western blot analysis
Corresponding Organization : Washington University in St. Louis
Other organizations : University of California, Los Angeles
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Variable analysis
independent variables
- Mouse anti-HA monoclonal antibody HA.11 (Covance; 1:500 WB)
- Rabbit polyclonal anti-HA SG77 (ThermoFisher; 1:500 IFA and WB)
- Mouse anti-Flag monoclonal antibody clone M2 (Sigma; 1:300 IFA)
- Mouse anti-EXP1 monoclonal antibody (Lisewski et al., 2014 (link)) (1:500 WB)
- Mouse anti-EXP2 monoclonal antibody clone 7.7 (Hall et al., 1983 (link)) (1:500 IFA and WB)
- Rabbit polyclonal anti-SBP1 (Blisnick et al., 2000 (link)) (1:500 IFA)
- Mouse anti-cMYC monoclonal antibody 9E10 (ThermoFisher; 1:166 IFA and WB)
- Rabbit polyclonal anti-Plasmodium Aldolase ab207494 (Abcam; 1:500 WB)
- Rabbit polyclonal anti-RFP R10367 (ThermoFisher; 1:500 WB)
- Immunofluorescence assays (IFA)
- Western blot (WB) analysis
- Dilutions of the specified antibodies
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