Molecular Biomarkers in Breast Cancer

BCa cells were lysed for Western blotting and proteins were detected using the antibodies, GAPDH (Santa Cruz 32233), P-Ser473 AKT (Cell Signaling 4060), INPP4B (Santa Cruz 12318), Total AKT (Cell Signaling 9272), ERα (produced in our lab, SC1-1), and p27 (Santa Cruz 528). Tissue fixation and sectioning were processed as previously described [36 (link), 73 (link)]. Tissue sections were incubated with ERα antibodies (Santa Cruz, MC-20), P-Ser473 AKT (Cell Signaling 4060), INPP4B (Abcam EPR3108), Ki67 (Novocastra).

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Hsu I., Yeh C.R., Slavin S., Miyamoto H., Netto G.J., Muyan M., Wu X.R., Messing E.M., Guancial E.A, & Yeh S. (2014). Estrogen Receptor Alpha Prevents Bladder Cancer Development via INPP4B inhibited Akt Pathway in vitro and in vivo. Oncotarget, 5(17), 7917-7935.

Publication 2014

GAPDH P-Ser473 AKT INPP4B Total AKT

Antibodies Cell Gapdh Inpp4b Proteins Tissue Tissue fixation

Corresponding Organization : University of Rochester Medical Center

Other organizations : Johns Hopkins Hospital, New York University

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Variable analysis

independent variables

Tissue fixation and sectioning process (previously described in references 36 and 73)

dependent variables

Protein expression levels of GAPDH, P-Ser473 AKT, INPP4B, Total AKT, ERα, and p27 in BCa cells (measured by Western blotting)
Immunohistochemical staining of ERα, P-Ser473 AKT, INPP4B, and Ki67 in tissue sections

control variables

BCa cell line used (not explicitly mentioned)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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