This experimental study was approved by the local ethics committee for animal
experiments (Dollvet Veterinary Vaccines Biological Substance Drug Production
Industry and Trade Inc. Şanlıurfa, Turkey). The experiments were
conducted on 30 three-month-old Wistar albino rats that ranged in weight from
200 g to 250 g. All the animals were kept under standard conditions and were
treated according to the guidelines of the National Institutes of Health. The
animals were kept in a 12h light/dark cycle (lights were turned on at 6 am). The
animals were deprived of food and water for 12h before surgery. The experiments
were conducted at the Dollvet Laboratory of Experimental Research.
The rats were randomly divided into three experimental groups: sham (n=10),
control (n=10) (I/R), and silymarin (n=10) (I/R-silymarin). They were
anesthetized intraperitoneally with xylazine (10 mg/kg) and ketamine
hydrochloride (50 mg/kg). The abdomens of the animals were shaved and then
cleaned with a povidone-iodine solution, and an abdominal midline incision was
made. In the sham group, only a laparotomy was carried out. In the control
group, a cross clamp was placed on the supraceliac aorta and ischemia was
applied for 45 min; this was followed by reperfusion for 60 min. In the
silymarin group, the cross-clamp was placed on the supraceliac aorta for 45 min,
and this was followed by reperfusion for 60 min. Silymarin was administered
during ischemia at a dose of 200 mg/kg via the intraperitoneal
route[6 (link)]. At the end of the study, blood samples were
obtained from the inferior vena cava of the rats, and heart, lung, liver and
kidney tissues were sampled and placed in formalin for pathological examination.
The blood samples obtained were centrifuged, and plasma was separated and stored
in the freezer at -80°C until biochemical analyses were performed. Silymarin
tablets were obtained from the pharmacy (Sigma-Aldrich, St. Louis, MO, USA). A
silymarin tablet of 85% purity was dissolved with 1% dimethyl sulfoxide before
it was administered.
experiments (Dollvet Veterinary Vaccines Biological Substance Drug Production
Industry and Trade Inc. Şanlıurfa, Turkey). The experiments were
conducted on 30 three-month-old Wistar albino rats that ranged in weight from
200 g to 250 g. All the animals were kept under standard conditions and were
treated according to the guidelines of the National Institutes of Health. The
animals were kept in a 12h light/dark cycle (lights were turned on at 6 am). The
animals were deprived of food and water for 12h before surgery. The experiments
were conducted at the Dollvet Laboratory of Experimental Research.
The rats were randomly divided into three experimental groups: sham (n=10),
control (n=10) (I/R), and silymarin (n=10) (I/R-silymarin). They were
anesthetized intraperitoneally with xylazine (10 mg/kg) and ketamine
hydrochloride (50 mg/kg). The abdomens of the animals were shaved and then
cleaned with a povidone-iodine solution, and an abdominal midline incision was
made. In the sham group, only a laparotomy was carried out. In the control
group, a cross clamp was placed on the supraceliac aorta and ischemia was
applied for 45 min; this was followed by reperfusion for 60 min. In the
silymarin group, the cross-clamp was placed on the supraceliac aorta for 45 min,
and this was followed by reperfusion for 60 min. Silymarin was administered
during ischemia at a dose of 200 mg/kg via the intraperitoneal
route[6 (link)]. At the end of the study, blood samples were
obtained from the inferior vena cava of the rats, and heart, lung, liver and
kidney tissues were sampled and placed in formalin for pathological examination.
The blood samples obtained were centrifuged, and plasma was separated and stored
in the freezer at -80°C until biochemical analyses were performed. Silymarin
tablets were obtained from the pharmacy (Sigma-Aldrich, St. Louis, MO, USA). A
silymarin tablet of 85% purity was dissolved with 1% dimethyl sulfoxide before
it was administered.
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