Histone-Induced Cytotoxicity Assay in Rat Cardiomyocytes

LDH assay was used to detect the cytotoxicity levels of histones on rat CMs according to the protocol which we described before [33 (link)]. Briefly, supernatants fluids were obtained from CMs exposed to histone, using phenol red-free media (Gibco, Grand Island, NY). The percentage of cytotoxicity (LDH release) from the sample treated with histone was measured compared to LDH content in total lysis fluids induced by 0.1% triton detergent (Sigma-Aldrich, St. Louis, MO). LDH release was measured using LDH assay kit (Cayman Chemical, Ann Arbor, MI) according to the manufacturer's instructions.

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Fattahi F., Russell M.W., Malan E.A., Parlett M., Abe E., Zetoune F.S, & Ward P.A. (2018). Harmful Roles of TLR3 and TLR9 in Cardiac Dysfunction Developing during Polymicrobial Sepsis. BioMed Research International, 2018, 4302726.

Publication 2018

phenol red-free media LDH assay kit

Assay Cayman Cytotoxicity Detergent Histone

Corresponding Organization : University of Michigan–Ann Arbor

Top 5 similar protocols

Variable analysis

independent variables

Histones

dependent variables

Cytotoxicity levels (LDH release)

control variables

Rat CMs (cardiomyocytes)
Phenol red-free media (Gibco, Grand Island, NY)
0.1% triton detergent (Sigma-Aldrich, St. Louis, MO)
LDH assay kit (Cayman Chemical, Ann Arbor, MI)

controls

Positive control: Total lysis fluids induced by 0.1% triton detergent
Negative control: Not explicitly mentioned

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